Yeast (Saccharomyces Cerevisiae): Difference between revisions
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[[File:S cerevisiae under DIC microscopy.jpg|thumb|''S. cerevisiae'', [[Differential interference contrast microscopy|differential interference contrast]] image]] | [[File:S cerevisiae under DIC microscopy.jpg|thumb|''S. cerevisiae'', [[Differential interference contrast microscopy|differential interference contrast]] image]] | ||
[[Image:20100911 232323 Yeast Live.jpg|thumb|''Saccharomyces cerevisiae''<br />Numbered ticks are 11 micrometers apart.]] | [[Image:20100911 232323 Yeast Live.jpg|thumb|''Saccharomyces cerevisiae''<br />Numbered ticks are 11 micrometers apart.]] | ||
When researchers look for an organism to use in their studies, they look for several traits. Among these are size, generation time, accessibility, manipulation, genetics, conservation of mechanisms, and potential economic benefit. The yeast species '' | When researchers look for an organism to use in their studies, they look for several traits. Among these are size, generation time, accessibility, manipulation, genetics, conservation of mechanisms, and potential economic benefit. The yeast species ''S. pombe'' and ''S. cerevisiae'' are both well studied; these two species diverged approximately 600 to 300 million years ago, and are significant tools in the study of DNA damage and repair mechanisms.<ref>{{cite book |first1= Jac A. |last1=Nickoloff |first2=James E. |last2=Haber |date=2011 |chapter=Mating-Type Control of DNA Repair and Recombination in ''Saccharomyces cerevisiae'' |doi=10.1007/978-1-59259-095-7_5 |pages=107–124 |editor1-first=Jac A. |editor1-last=Nickoloff |editor2-first=Merl F. |editor2-last=Hoekstra |title=DNA Damage and Repair |series=Contemporary Cancer Research |isbn=978-1-59259-095-7|doi-broken-date=1 August 2023 | chapter-url=https://link.springer.com/chapter/10.1007/978-1-59259-095-7_5}}</ref> | ||
''S. cerevisiae'' has developed as a [[model organism]] because it scores favorably on a number of these criteria. | ''S. cerevisiae'' has developed as a [[model organism]] because it scores favorably on a number of these criteria. | ||
* As a single-cell organism, ''S. cerevisiae'' is small with a short generation time (doubling time 1.25–2 hours<ref>{{cite book |editor=Boekhout, T. |editor2=Robert, V. |date=2003 |title=Yeasts in Food: Beneficial and Detrimental aspects |publisher=Behr's Verlag |isbn=978-3-86022-961-3 |page=322 |url=https://books.google.com/books?id=GG-60Vtl81EC |access-date=January 10, 2011}}</ref> at {{convert|30|C|F|disp=or}}) and can be easily | * As a single-cell organism, ''S. cerevisiae'' is small with a short generation time (doubling time 1.25–2 hours<ref>{{cite book |editor=Boekhout, T. |editor2=Robert, V. |date=2003 |title=Yeasts in Food: Beneficial and Detrimental aspects |publisher=Behr's Verlag |isbn=978-3-86022-961-3 |page=322 |url=https://books.google.com/books?id=GG-60Vtl81EC |access-date=January 10, 2011}}</ref> at {{convert|30|C|F|disp=or}}) and can be easily cultured. These are all positive characteristics in that they allow for the swift production and maintenance of multiple specimen lines at low cost. | ||
* ''S. cerevisiae'' divides with meiosis, allowing it to be a candidate for sexual genetics research. | * ''S. cerevisiae'' divides with meiosis, allowing it to be a candidate for sexual genetics research. | ||
* ''S. cerevisiae'' can be | * ''S. cerevisiae'' can be transformed allowing for either the addition of new genes or deletion through homologous recombination. Furthermore, the ability to grow ''S. cerevisiae'' as a haploid simplifies the creation of gene knockout strains. | ||
* As a | * As a eukaryote, ''S. cerevisiae'' shares the complex internal cell structure of plants and animals without the high percentage of non-coding DNA that can confound research in higher eukaryotes. | ||
* ''S. cerevisiae'' research is a strong economic driver, at least initially, as a result of its established use in industry. | * ''S. cerevisiae'' research is a strong economic driver, at least initially, as a result of its established use in industry. | ||
== References == | == References == | ||
<references /> | <references /> | ||
Revision as of 02:21, 8 December 2023
Saccharomyces cerevisiae (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungus microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have been originally isolated from the skin of grapes. It is one of the most intensively studied eukaryotic model organisms in molecular and cell biology, much like Escherichia coli as the model bacterium. S. cerevisiae cells are round to ovoid, 5–10 μm in diameter. It reproduces by budding.[1]
Model organism
S. cerevisiae, differential interference contrast image
When researchers look for an organism to use in their studies, they look for several traits. Among these are size, generation time, accessibility, manipulation, genetics, conservation of mechanisms, and potential economic benefit. The yeast species S. pombe and S. cerevisiae are both well studied; these two species diverged approximately 600 to 300 million years ago, and are significant tools in the study of DNA damage and repair mechanisms.[2]
S. cerevisiae has developed as a model organism because it scores favorably on a number of these criteria.
- As a single-cell organism, S. cerevisiae is small with a short generation time (doubling time 1.25–2 hours[3] at 30 °C or 86 °F) and can be easily cultured. These are all positive characteristics in that they allow for the swift production and maintenance of multiple specimen lines at low cost.
- S. cerevisiae divides with meiosis, allowing it to be a candidate for sexual genetics research.
- S. cerevisiae can be transformed allowing for either the addition of new genes or deletion through homologous recombination. Furthermore, the ability to grow S. cerevisiae as a haploid simplifies the creation of gene knockout strains.
- As a eukaryote, S. cerevisiae shares the complex internal cell structure of plants and animals without the high percentage of non-coding DNA that can confound research in higher eukaryotes.
- S. cerevisiae research is a strong economic driver, at least initially, as a result of its established use in industry.