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EC50: Difference between revisions
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{{DISPLAYTITLE:EC<sub>50</sub>}} | {{DISPLAYTITLE:EC<sub>50</sub>}} | ||
[[File:Dose_response_antagonist.jpg|right|thumb|400px|The tissue response (y-axis) to an agonist, in log concentration (x-axis), in the presence of different antagonist concentrations. The EC<sub>50</sub> of the agonist is represented by the x co-ordinate that corresponds with the half-maximum of the leftmost curve. This is denoted by [A]]] | [[File:Dose_response_antagonist.jpg|right|thumb|400px|The tissue response (y-axis) to an agonist, in log concentration (x-axis), in the presence of different antagonist concentrations. The EC<sub>50</sub> of the agonist is represented by the x co-ordinate that corresponds with the half-maximum of the leftmost curve. This is denoted by [A]]] | ||
'''Half maximal effective concentration''' ('''EC<sub>50</sub>''') is a measure of the concentration of a drug, antibody or toxicant which induces a biological response halfway between the baseline and maximum after a specified exposure time.<ref>{{cite web|url=http://www.graphpad.com/curvefit/introduction89.htm |title=Introducing dose response curves |archive-url=https://web.archive.org/web/20120730150339/http://www.graphpad.com/curvefit/introduction89.htm |archive-date=2012-07-30 |publisher=Graphpad Software}}</ref> More simply, EC<sub>50</sub> can be defined as the ''concentration required to obtain a 50% [...] effect'' | '''Half maximal effective concentration''' ('''EC<sub>50</sub>''') is a measure of the concentration of a drug, antibody or toxicant which induces a biological response halfway between the baseline and maximum after a specified exposure time.<ref>{{cite web|url=http://www.graphpad.com/curvefit/introduction89.htm |title=Introducing dose response curves |archive-url=https://web.archive.org/web/20120730150339/http://www.graphpad.com/curvefit/introduction89.htm |archive-date=2012-07-30 |publisher=Graphpad Software}}</ref> More simply, EC<sub>50</sub> can be defined as the ''concentration required to obtain a 50% [...] effect''{{pmid|23265506}} and may be also written as [A]<sub>50</sub>.{{pmid|14657418}} It is commonly used as a measure of a drug's potency, although the use of EC<sub>50</sub> is preferred over that of 'potency', which has been criticised for its vagueness.{{pmid|14657418}} EC<sub>50</sub> is a measure of concentration, expressed in molar units (M), where 1 M is equivalent to 1 mol/L. | ||
The EC<sub>50</sub> of a ''graded'' dose response curve therefore represents the concentration of a compound where 50% of its maximal effect is observed.<ref>{{cite web|url=http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-E/EC50.html|title=Northwestern Molbiosci EC50 definition|access-date=2019-05-27}}</ref> | The EC<sub>50</sub> of a ''graded'' dose response curve therefore represents the concentration of a compound where 50% of its maximal effect is observed.<ref>{{cite web|url=http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-E/EC50.html|title=Northwestern Molbiosci EC50 definition|access-date=2019-05-27}}</ref> | ||